Real Time PCR Reagents and Kits
Products designed for use during real-time (quantitative) PCR procedures. Includes master mixes, DNA polymerases, reverse transcriptases, size standards, kits, and reagents.
Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique that combines the reverse transcription of RNA into DNA and the subsequent amplification of the DNA using PCR.
In RT-PCR, a reverse transcriptase enzyme converts an RNA template to its complementary DNA (cDNA). The cDNA then becomes the template for PCR amplification.
A typical RT-PCR kit includes the main components needed for the assay:
- Buffer
- Primers – a combination of deoxynucleotide triphosphates (dNTPs) of the four bases of adenine (dATP), cytosine (dCTP), guanine (dGTP), and thymine (dTTP)
- Reverse transcriptase reagent
The combination of RT-PCR and qPCR techniques (sometimes labelled RT-qPCR) is often used in research and clinical labs to analyze gene expression and quantify viral RNA. Since this method uses fluorescence to track the amplification reaction, these kits may also include:
- Fluorogenic primers or labeled fluorogenic probes
- Fluorescent dye
Reagents for cDNA PCR can be purchased separately or as cDNA synthesis kits.
RT-PCR is commonly used for RNA detection, molecular cloning, and gene sequencing. RT-qPCR is used for gene expression analysis, RNAi or microarray validation, pathogen detection, genetic testing, and other disease research.
Sciencell Research Laboratories Telomerase Activity Quantification qPCR Assay Kit, 100 reactions, SYBRGreen dye-based, Hot-start property
Telomerase Activity Quantification qPCR Assay Kit, 100 reactions, SYBRGreen dye-based, Hot-start property
Bioss Rhinovirus
Rhinovirus Primary Antibody Unconjugated Host Species Mouse Target Species Clonality Monoclonal Applications ELISA Concentration 100 g/ml Size 100 ug Storage Buffer PBS (pH 7 2) with 0 1% azide
Biotium PMA Real-Time PCR Bacterial Viability Kit - Listeria monocytogenes (hly) PMAxx™
This PMAxx™-PCR kit is designed for selective detection of viable bacteria from a specific strain using PMAxx™ dye and real-time PCR. This kit provides primers for specific amplification of the Listeria monocytogenes hly gene. The kit has sufficient reagents for treating of 80 bacterial cultures and performing 200 PCR reactions with PMAxx™. PMAxx™ is a new and improved version of PMA designed by Biotium scientists to be a superior alternative to PMA. While PMA is generally effective at differentiating between live and dead bacteria by qPCR, it does not completely eliminate PCR products from dead cell DNA. This could potentially give false positive results. Biotium’s new dye PMAxx™ is much more effective at eliminating PCR amplification of dead cell DNA, and therefore provides the best discrimination between live and dead bacteria. Listeria monocytogenes is a food-borne pathogen that can cause digestive illness and fetal harm.
TRIBIOSCIENCE INC Bacillus Subtilis qPCR Kit 100 Reactions
Tribioscience’s Bacillus Subtilis qPCR Kit includes bacillus subtilis positive and negative controls, PCR internal controls labeled with Hex, a qPCR super mix, and the primer-probe mix in which the probe has labeled with FAM for the target gene. These aid in a straightforward interpretation of the results. Applications - Detect bacillus-derived DNA in plant, cannabis, cannabis ingredients, grain, food, herbals, and animal feed. Key Features - High sensitivity and specificity for all bacillus subtilis species. High efficiency - the optimal systemic conditions for PCR amplifications. Streamlined protocol - just add DNA Template and water.
ACROBiosystems E. coli resDNA Quantitative Kit (qPCR)
Residual host cell DNA refers to fragments of DNA from the host organism that are left behind after a biological process such as the production of a biopharmaceutical product or the cultivation of a cell line. This residual DNA can potentially contaminate the final product and affect its safety and efficacy. In the context of biopharmaceutical production, regulatory agencies such as the FDA and EMA have set limits on the amount of residual host cell DNA that is acceptable in a final product. These limits vary depending on the type of product and the route of administration, and residue host cell DNA quantitative kits are designed to ensure that the final product is safe for human use.
Meridian Life Science Lyo-Ready qPCR Mix 2.6x, 8ML
Lyo-Ready qPCR Mix, 2.6x is a higher concentration, glycerol-free formulation that contains Taq polymerase, reaction buffer, dNTP, MgCl2 and lyo-excipients. It is ideal for developing room temperature, lyophilized, multiplex qPCR diagnostic tests and is suited for use with microfluidic qPCR, where the initial volume needs to be smaller. It can be used as a wet mix or it can be lyophilized without impacting its performance post rehydration.
Alkali Scientific Radiant™ Probe Lo-ROX qPCR Kit, 500 Reactions, (5 x 1mL)
Alkali Scientific offers Radiant™ Probe Lo-ROX qPCR Kits, a high-performance portfolio of 2X ready-to-use real-time PCR reagents designed for best-in-class quantitative PCR. Radiant™ qPCR Kits are engineered for robust real-time PCR with earlier quantification cycle values (Ct), industry-leading sensitivity (increased limit of detection) and exceptional speed (rapid extension rates). The proprietary buffer system allows for highly efficient amplification of GC-rich and AT-rich sequences in addition to dramatic improvements in PCR sensitivity in low-copy assays and PCR conditions conducive to ultra-fast amplification. Furthermore, Radiant™ Probe Lo-ROX qPCR Kits are optimized for advanced multiplexing capability, delivering the same high efficiency, early Ct, and reaction speed even in complex multiplex assays.
Mayflower Bioscience HOTFIREPol EvaGreen qPCR ROX
HOT FIREPol Probe qPCR Mix Plus (ROX) is optimized for real-time quantitative PCR assays and contains all the components necessary to perform qPCR, with the exception of template, primers, and probe. The qPCR Mix contains optimized components and HOT FIREPol DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets. HOT FIREPol Probe qPCR Mix Plus (ROX) is optimized for DNA hydrolysis probes based on the 5´ flap endonuclease activity. HOT FIREPol DNA Polymerase is activated by a 12 min incubation step at 95°C. This prevents the extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.
Agilent Technologies Brilliant II QRT-PCR Master Mix, 1-Step, 400 reactions, 25 μl/reaction, Moloney-based RT, 50°C, 5.5 mM MgCl2, multiplexing: true, storage: -20°C, stability: 6 months
Brilliant II QRT-PCR Master Mix, 1-Step, 400 reactions, 25 μl/reaction, Moloney-based RT, 50°C, 5.5 mM MgCl2, multiplexing: true, storage: -20°C, stability: 6 months
XpressBio XpressMyco qPCR Mycoplasma Detection Kit, 100 Reactions
The XpressMyco qPCR Mycoplasma Detection Kit is a real-time PCR (qPCR) assay for rapid, quantitative detection of mycoplasma contaminants in cell culture.
LUNA NANOTECH INC Streptavidin Magnetic Beads, 2 ML
Streptavidin Coated Magnetic Beads, 500 nm Diameter. 2 mL @ 10 mg/mL
500 nm diameter magnetic beads with Streptavdin protein functionalized surface.
Features:
- High binding capacity of Biotin-conjugated molecules:
- 2,000 to 4,000 pmole of biotin / mg of magnetic beads
- 15-20 µg botinylated antibody / mg of magnetic beads
- Rapid and efficient biomolecule purification from complex samples.
- Fast response to a magnet.
- High monodispersity.
Applications:
Magnetic separation of Biotinylated cells, DNA, proteins, antibodies, and small ligands from complex samples. The streptavidin-coated magnetic beads are simply added to Biotinylated molecules for binding. The samples are transferred into a magnetic rack for easy removal of unbound residue.
Citations:
J. H. Molinski, S. Parwal and J. X. J. Zhang, "Laser-Manufactured
ACROBiosystems HEK293 resDNA Quantitation Kit (qPCR)
Residual host cell DNA refers to fragments of DNA from the host organism that are left behind after a biological process such as the production of a biopharmaceutical product or the cultivation of a cell line. This residual DNA can potentially contaminate the final product and affect its safety and efficacy. In the context of biopharmaceutical production, regulatory agencies such as the FDA and EMA have set limits on the amount of residual host cell DNA that is acceptable in a final product. These limits vary depending on the type of product and the route of administration, and residue host cell DNA quantitative kits are designed to ensure that the final product is safe for human use.
New England Biolabs, Inc. Luna Probe One-Step RT-qPCR 4X Mix with UDG (No ROX) 2000 reactions
The Luna Probe One-Step RT-qPCR Mix with UDG (No ROX) is supplied at a 4X concentration and enables higher amounts of sample input which is relevant for applications where RNA present in low abundance is of interest such as pathogen detection. The mix consolidates the components for one-step RT-qPCR into a single tube including Luna WarmStart RT Hot Start Taq DNA Polymerase dNTPs and Murine RNase Inhibitor in an optimized buffer. Combining Hot Start Taq DNA Polymerase with a novel WarmStart-activated reverse transcriptase allows for dual control of enzyme activity via reversible aptamer-based inhibition. This temperature-dependent activation helps to prevent undesirable non-specific priming and extension prior to thermocycling providing added security for setting up reactions at room temperature. This formulation contains no reference dye and is compatible with any instrument that does not require ROX (if ROX normalization is needed ROX can be added.)